![]() Ent Cx, entorhinal cortex DG, dentate gyrus. TdTomato-expressing PNs in CA1 are indicated by white arrows. ( C2) Optical slice extracted from the same brain as in C1, with focus of hippocampal CA1 area. Cb, cerebellum CPu, caudate putamen Hip, hippocampus Cx, cortex scale bar: 100 µm. Green fluorescent protein Syto16 is used to visualize brain structures. In red, fate-mapped glutamatergic neurons within hippocampus (Hip) and deep layers of cortex (Cx). ( C1) Two-dimensional view of the 3D reconstruction of a clarified hemisphere obtained by light-sheet microscopy from a Neurog2 CreER-Tdt mouse induced at E12.5. ( C1-2) Quantification of cell abundance of fate-mapped CA1PNs. ( B2) Cumulative fraction of E12.5, E14.5, and E16.5 PNs in ventral CA1. In turn, E14.5PNs also occupy deeper positions than E16.5 PNs (p<0.0001, CI 95% ). ( B1) Cumulative fraction of E12.5, E14.5 and E16.5 PNs in dorsal CA1, calculated as distance in µm from the superficial (lower) border of the stratum pyramidale. Gray shaded areas represent the thickness of the stratum pyramidale. ( B1-2) Quantification of the soma location distribution of fate-mapped CA1 PNs. Cx: Cortex DG: dentate gyrus so: stratum oriens sp: stratum pyramidale sr: stratum radiatum slm: stratum lacunosum-moleculare. Note that in the above cortical areas, E12.5 PNs are restricted to the deepest layers, E14.5 PNs the middle ones (IV-III) and E16.5 PNs are most superficial (layers I-II). E12.5 PNs are rare and dispersed, while E14.5 and E16.5 are predominantly found in the deep (upper) and superficial (lower) portion of the pyramidal layer, respectively. ( A1-3) Top, representative sections of the dorsal hippocampus and cortex, illustrating Tdtomato (Tdt) labeling in CA1 pyramidal neurons (PNs) in Neurog2 CreER-Tdt mice after tamoxifen induction at embryonic day 12.5 (E12.5), E14.5, and E16.5, from left to right. ![]()
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